Bioaccumulation, elimination, and toxic effect of cadmium on structure of gills and hepatopancreas of freshwater prawn macrobrachium sintangese (De Man, 1898)

Soegianto, A.^a ,

Winarni, D.^a,

Handayani, U.S.^b,

Hartati^b

^a  Department of Biology, Faculty of Sciences and Technology, Airlangga University, Jl. Mulyorejo, Surabaya 60115, Indonesia ^b  

Abstract

The objectives of this study were to determine the acute toxicity of cadmium and to examine the bioaccumulation and elimination of cadmium in different tissues of the freshwater prawn Macrobrachium sintangese. It also evaluated the structural damage of gills and hepatopancreas of M. sintangese when administered to sublethal cadmium concentration and when exposed prawns were transferred to cadmium-free media. According to the mortality data, the 96 h LC₅₀ value of Cd to M. sintangese was 86 μg/L. The highest cadmium accumulation was observed in gills, followed by the hepatopancreas, and the abdominal muscle. After being transferred to cadmium-free media, the highest cadmium elimination was observed in abdominal muscle, followed by the gills and hepatopancreas. The gills of prawns exposed to cadmium exhibited a severe hyperplasia, vacuolization, and multiple necroses which resulted to the swelling of lamellae. After transferring the cadmium-exposed prawns into the control media, the histopathological effects decreased. Severe alterations to the hepatopancreatic tissue were observed in prawns exposed to cadmium. The tubular epithelial cells were heavily vacuolated and even ruptured. The number of large vacuoles and R cells appeared in the tubular epithelial cells of the hepatopancreas. After transferring to the control media, the histological alterations of the hepatopancreas decreased. The tubular epithelial cells began to rearrange to the normal structure. The number of R cells and B cells were noted in the epithelial cells. The thickness of tubular epithelial cells was comparable to the controls. Due to the sensitivity of M. sintangese to cadmium, therefore this species potentially can be used as a test organism in toxicity assays. © 2013 Springer Science+Business Media Dordrecht.

Author keywords

Bioaccumulation; Cadmium; Elimination; Gills; Hepatopancreas; Macrobrachium sintangese

Indexed keywords

Abdominal muscle; Elimination; Epithelial cells; Gills; Hepatopancreas; Macrobrachium sintangese; Normal structure; Structural damages

Engineering controlled terms: Bioaccumulation; Biochemistry; Cells; Muscle; Shellfish; Toxicity; Water

Engineering main heading: Cadmium

EMTREE drug terms: cadmium

GEOBASE Subject Index: bioaccumulation; cadmium; concentration (composition); crustacean; histopathology; mortality; toxicity

EMTREE medical terms: abdominal wall musculature; acute toxicity; anatomical variation; animal tissue; article; bioaccumulation; bioassay; cadmium poisoning; cell hyperplasia; cell swelling; cell vacuole; concentration (parameters); controlled study; disease severity; elimination reaction; freshwater species; gill; hepatopancreas; histopathology; kidney tubule cell; lamellar body; Macrobrachium; Macrobrachium sintangese; nonhuman; sensitivity analysis; tissue level; tissue necrosis

Species Index: Decapoda (Crustacea); Macrobrachium

Full Text :

http://link.springer.com/article/10.1007%2Fs11270-013-1575-4